Determination of Opiates and Cocaine and Its Metabolites in Biological Fluids by High-Performance Liquid Chromatography with Electrospray Tandem Mass Spectrometry | Zendy

A Cailleux | Zendy; Anne Le Bouil | Zendy; B. Auger | Zendy; G. Bonsergent | Zendy; Alain Turcant | Zendy; Philippe Allain | Zendy

Open Access

Determination of Opiates and Cocaine and Its Metabolites in Biological Fluids by High-Performance Liquid Chromatography with Electrospray Tandem Mass Spectrometry

Author(s) -

A Cailleux,

Anne Le Bouil,

B. Auger,

G. Bonsergent,

Alain Turcant,

Philippe Allain

Publication year - 1999

Publication title -

journal of analytical toxicology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.161

H-Index - 76

eISSN - 1945-2403

pISSN - 0146-4760

DOI - 10.1093/jat/23.7.620

Subject(s) - chromatography , chemistry , electrospray ionization , tandem mass spectrometry , mass spectrometry , electrospray , detection limit , selected reaction monitoring , urine , liquid chromatography–mass spectrometry , extraction (chemistry) , coefficient of variation , biochemistry

A rapid, sensitive, and specific method for the determination of opiates and cocaine and metabolites in urine, plasma, and blood was established. A one-step extraction followed by liquid chromatography-electrospray ionization tandem mass spectrometry operating in multiple reaction monitoring mode was used. Two chromatographic runs were performed, each in less than 6 min. The lower limit for accurate quantitative determination was 5 microg/L for cocaine and metabolites and 10 microg/L for opiates. Linearity was obtained from 10 to 1000 microg/L. Intraday (n = 6) and interday (n = 6) precisions and recoveries (n = 6) were determined at 10 or 25, 100, and 1000 microg/L concentrations. Precisions with a coefficient of variation less than 15% were obtained. Recoveries between 85 and 115% were determined.

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