Open AccessHPLC Determination of Flumethrin, Deltamethrin, Cypermethrin, and Cyhalothrin Residues in the Milk and Blood of Lactating Dairy Cows
Author(s) -
Denise Zuccari Bissacot,
I. Vassilieff
Publication year - 1997
Publication title -
journal of analytical toxicology.
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.161
H-Index - 76
eISSN - 1945-2403
pISSN - 0146-4760
DOI - 10.1093/jat/21.5.397
Subject(s) - deltamethrin , cypermethrin , pyrethroid , chromatography , chemistry , cyhalothrin , residue (chemistry) , hexane , high performance liquid chromatography , pesticide residue , pesticide , biology , biochemistry , agronomy
A procedure to determine residue concentrations of synthetic pyrethroid insecticides (flumethrin, deltamethrin, cypermethrin and cyhalothrin) in the milk and blood of lactating dairy cows was developed. Extraction was performed with acetoritrile, n-hexane partitioning, and silica gel column cleanup with n-hexane and diethyl ether. Analysis was carried out by high-performance liquid chromatography and ultraviolet detection. Recovery of the four pyrethroids averaged 78 to 91% with a minimum detectable concentration of 0.001 mg/kg. The method was reproducible and sensitive.