Open AccessA Simple and Sensitive Quantitation of N,N-Dimethyltryptamine by Gas Chromatography with Surface Ionization Detection
Author(s) -
A. Ishii,
Hiroshi Seno,
Osamu Suzuki,
Hideki Hattori,
Takeshi Kumazawa
Publication year - 1997
Publication title -
journal of analytical toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.161
H-Index - 76
eISSN - 1945-2403
pISSN - 0146-4760
DOI - 10.1093/jat/21.1.36
Subject(s) - chromatography , detection limit , calibration curve , chemistry , gas chromatography , flame ionization detector , cartridge , urine , extraction (chemistry) , solid phase extraction , materials science , biochemistry , metallurgy
A simple and sensitive method for determination of N,N-dimethyltryptamine (DMT) by gas chromatography (GC) with surface ionization detection (SID) is presented. Whole blood or urine, containing DMT and gramine (internal standard), was subjected to solid-phase extraction with a Sep-Pak C18 cartridge before analysis by GC-SID. The calibration curve was linear in the DMT range of 1.25-20 ng/mL blood or urine. The detection limit of DMT was about 0.5 ng/mL (10 pg on-column). The recovery of both DMT and gramine spiked in biological fluids was above 86%.
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