Open AccessInvestigation of the Effects of Solution Composition and Container Material Type on the Loss of 11-nor- 9-THC-9-Carboxylic Acid
Author(s) -
Kenneth D. Roth,
N. A. Siegel,
Robert W. Johnson,
L. Litauszki,
Lawrence Salvati,
Charles A. Harrington,
L. K. Wray
Publication year - 1996
Publication title -
journal of analytical toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.161
H-Index - 76
eISSN - 1945-2403
pISSN - 0146-4760
DOI - 10.1093/jat/20.5.291
Subject(s) - chemistry , polyethylene , diluent , x ray photoelectron spectroscopy , polypropylene , chromatography , polystyrene , polyvinylpyrrolidone , analytical chemistry (journal) , nuclear chemistry , organic chemistry , polymer , chemical engineering , engineering
The loss of 11-nor-delta 9-tetrahydrocannabinol-9-carboxylic acid (THC-COOH) from solution was studied using fluorescence polarization immunoassay (FPIA) technology and x-ray photoelectron spectroscopy (XPS). Several materials (glass, silylated glass, high density polyethylene, polypropylene, polystyrene, polymethylmethacrylate, Teflon, and Kynar) were studied along with three solvents (water, urine, and Abbott cannabinoids diluent). THC-COOH losses ranging from 0 to 9.7 ng/cm2 and concentration reductions to 46% of starting values were measured. XPS indicated the presence of fluorine-labeled THC-COOH at materials surfaces. A half-life of 10 min was calculated for THC-COOH loss from urine stored in high density polyethylene at room temperature. Sample handling losses during pipetting were determined and ranged from 1.1 to 7.9 ng per aliquot. The effects of sample volume and sample handling on the THC-COOH concentrations of controls were also investigated.
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