Optimization of the MALDIxin test for the rapid identification of colistin resistance in Klebsiella pneumoniae using MALDI-TOF MS | Zendy

Laurent Dortet | Zendy; Agnieszka Broda | Zendy; Sandrine Bernabeu | Zendy; Y. Glupczynski | Zendy; Pierre Bogaerts | Zendy; Rémy A. Bonnin | Zendy; Thierry Naas | Zendy; Alain Filloux | Zendy; Gérald Larrouy-Maumus | Zendy

Open Access

Optimization of the MALDIxin test for the rapid identification of colistin resistance in Klebsiella pneumoniae using MALDI-TOF MS

Author(s) -

Laurent Dortet,

Agnieszka Broda,

Sandrine Bernabeu,

Y. Glupczynski,

Pierre Bogaerts,

Rémy A. Bonnin,

Thierry Naas,

Alain Filloux,

Gérald Larrouy-Maumus

Publication year - 2019

Publication title -

journal of antimicrobial chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.124

H-Index - 194

eISSN - 1460-2091

pISSN - 0305-7453

DOI - 10.1093/jac/dkz405

Subject(s) - colistin , klebsiella pneumoniae , acinetobacter baumannii , broth microdilution , microbiology and biotechnology , polymyxin , biology , escherichia coli , bacteria , antibiotics , gene , minimum inhibitory concentration , pseudomonas aeruginosa , biochemistry , genetics

With the dissemination of carbapenemase producers, a revival of colistin was observed for the treatment of infections caused by MDR Gram-negatives. Unfortunately, the increasing usage of colistin led to the emergence of resistance. In Klebsiella pneumoniae, colistin resistance arises through addition of 4-amino-l-arabinose (l-Ara4N) or phosphoethanolamine (pEtN) to the native lipid A. The underlying mechanisms involve numerous chromosome-encoded genes or the plasmid-encoded pEtN transferase MCR. Currently, detection of colistin resistance is time-consuming since it still relies on MIC determination by broth microdilution. Recently, a rapid diagnostic test based on MALDI-TOF MS detection of modified lipid A was developed (the MALDIxin test) and tested on Escherichia coli and Acinetobacter baumannii.

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