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Rapid sequencing of MRSA direct from clinical plates in a routine microbiology laboratory
Author(s) -
Beth Blane,
Kathy E. Raven,
Danielle Leek,
Nicholas M. Brown,
Julian Parkhill,
Sharon J. Peacock
Publication year - 2019
Publication title -
journal of antimicrobial chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.124
H-Index - 194
eISSN - 1460-2091
pISSN - 0305-7453
DOI - 10.1093/jac/dkz170
Subject(s) - clinical microbiology , dna sequencing , outbreak , microbiology and biotechnology , staphylococcal infections , dna extraction , whole genome sequencing , isolation (microbiology) , biology , methicillin resistant staphylococcus aureus , infection control , medicine , polymerase chain reaction , virology , staphylococcus aureus , genome , genetics , bacteria , dna , gene , pathology
Routine sequencing of MRSA could bring about significant improvements to outbreak detection and investigation. Sequencing is commonly performed using DNA extracted from a pure culture, but overcoming the delay associated with this step could reduce the time to infection control interventions.

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