ChloS-HRM, a novel assay to identify chloramphenicol-susceptible Escherichia coli and Klebsiella pneumoniae in Malawi | Zendy

Christopher T. Williams | Zendy; Patrick Musicha | Zendy; Nicholas Feasey | Zendy; Emily R. Adams | Zendy; Thomas Edwards | Zendy

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ChloS-HRM, a novel assay to identify chloramphenicol-susceptible Escherichia coli and Klebsiella pneumoniae in Malawi

Author(s) -

Christopher T. Williams,

Patrick Musicha,

Nicholas Feasey,

Emily R. Adams,

Thomas Edwards

Publication year - 2019

Publication title -

journal of antimicrobial chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.124

H-Index - 194

eISSN - 1460-2091

pISSN - 0305-7453

DOI - 10.1093/jac/dky563

Subject(s) - klebsiella pneumoniae , chloramphenicol , microbiology and biotechnology , enterobacteriaceae , escherichia coli , antimicrobial , biology , antibiotics , gene , genetics

Chloramphenicol is a broad-spectrum antimicrobial widely available in sub-Saharan Africa. With susceptibility re-emerging among Enterobacteriaceae in Blantyre, Malawi, we designed and evaluated a new high-resolution melt (HRM) RT-PCR assay, ChloS-HRM, to identify chloramphenicol-susceptible infections in a hospital setting.

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