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1 Yang YJ, Wu PJ, Livermore DM. Biochemical characterization of a b-lactamase that hydrolyzes penems and carbapenems from two Serratia marcescens isolates. Antimicrob Agents Chemother 1990; 34: 755–8. 2 Bush K, Pannell M, Lock JL et al. Detection systems for carbapenemase gene identification should include the SME serine carbapenemase. Int J Antimicrob Agents 2013; 41: 1–4. 3 Mataseje LF, Boyd DA, Delport J et al. Serratia marcescens harbouring SMEtype class A carbapenemases in Canada and the presence of blaSME on a novel genomic island, SmarGI1-1. J Antimicrob Chemother 2014; 69: 1825–9. 4 van der Zwaluw K, de HA, Pluister GN et al. The carbapenem inactivation method (CIM), a simple and low-cost alternative for the Carba NP test to assess phenotypic carbapenemase activity in Gram-negative rods. PLoS One 2015; 10: e0123690. 5 Doumith M, Day M, Ciesielczuk H et al. Rapid identification of major Escherichia coli sequence types causing urinary tract and bloodstream infections. J Clin Microbiol 2015; 53: 160–6. 6 Champion HM, Bennett PM, Lewis DA et al. Cloning and characterization of an AAC(60) gene from Serratia marcescens. J Antimicrob Chemother 1988; 22: 587–96. 7 Shaw KJ, Rather PN, Sabatelli FJ et al. Characterization of the chromosomal aac(60)-Ic gene from Serratia marcescens. Antimicrob Agents Chemother 1992; 36: 1447–55. 8 Pasteran F, Mendez T, Guerriero L et al. Sensitive screening tests for suspected class A carbapenemase production in species of Enterobacteriaceae. J Clin Microbiol 2009; 47: 1631–9. 9 AbdelGhani S, Thomson GK, Snyder JW et al. Comparison of the Carba NP, modified Carba NP, and updated Rosco Neo-Rapid Carb kit tests for carbapenemase detection. J Clin Microbiol 2015; 53: 3539–42. 10 Lefebvre B, Levesque S, Bourgault AM et al. Carbapenem non-susceptible Enterobacteriaceae in Quebec, Canada: results of a laboratory surveillance program (2010-2012). PLoS One 2015; 10: e0125076.

Ultradeep sequencing detection of the R263K integrase inhibitor drug resistance mutation