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To identify a novel putative lincosamide resistance gene determinant in a swine Enterococcus faecalis E531 exhibiting a lincosamide resistance/macrolide susceptibility (L R M S ) phenotype and to determine its location and genetic environment.

Novellnu(G) gene conferring resistance to lincomycin by nucleotidylation, located on Tn6260fromEnterococcus faecalisE531