PCR-based detection of Aspergillus fumigatus Cyp51A mutations on bronchoalveolar lavage: a multicentre validation of the AsperGenius assay ® in 201 patients with haematological disease suspected for invasive aspergillosis | Zendy

GaLai M. Chong | Zendy; Martha T. van der Beek | Zendy; Peter A. von dem Borne | Zendy; Jerina Boelens | Zendy; Eva Steel | Zendy; Greetje A. Kampinga | Zendy; Lambert F. R. Span | Zendy; Katrien Lagrou | Zendy; Johan Maertens | Zendy; Gijs Dingemans | Zendy; Giel R. Gaajetaan | Zendy; Dennis W. E. van Tegelen | Zendy; Jan J. Cornelissen | Zendy; Alieke G. Vonk | Zendy; Bart Rijnders | Zendy

Open Access

PCR-based detection of Aspergillus fumigatus Cyp51A mutations on bronchoalveolar lavage: a multicentre validation of the AsperGenius assay ^® in 201 patients with haematological disease suspected for invasive aspergillosis

Author(s) -

GaLai M. Chong,

Martha T. van der Beek,

Peter A. von dem Borne,

Jerina Boelens,

Eva Steel,

Greetje A. Kampinga,

Lambert F. R. Span,

Katrien Lagrou,

Johan Maertens,

Gijs Dingemans,

Giel R. Gaajetaan,

Dennis W. E. van Tegelen,

Jan J. Cornelissen,

Alieke G. Vonk,

Bart Rijnders

Publication year - 2016

Publication title -

journal of antimicrobial chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.124

H-Index - 194

eISSN - 1460-2091

pISSN - 0305-7453

DOI - 10.1093/jac/dkw323

Subject(s) - bronchoalveolar lavage , aspergillus fumigatus , aspergillosis , galactomannan , aspergillus , voriconazole , medicine , gastroenterology , azole , immunology , microbiology and biotechnology , biology , lung , antifungal

In patients with invasive aspergillosis (IA), fungal cultures are mostly negative. Consequently, azole resistance often remains undetected. The AsperGenius ® multiplex real-time PCR assay identifies clinically relevant Aspergillus species and four resistance-associated mutations (RAMs; TR34/L98H/T289A/Y121F) in the Cyp51A gene. This multicentre study evaluated the diagnostic performance of this assay on bronchoalveolar lavage (BAL) fluid and correlated the presence of RAMs with azole treatment failure and mortality.

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