Development of a Luminex xTAG®assay for cost-effective multiplex detection of β-lactamases in Gram-negative bacteria | Zendy

PieterJan Ceyssens | Zendy; Cristina Garcı́a-Graells | Zendy; Frédéric Fux | Zendy; Nadine Botteldoorn | Zendy; Wesley Mattheus | Zendy; Véronique Wuyts | Zendy; Sigrid C. J. De Keersmaecker | Zendy; Katelijne Dierick | Zendy; Sophie Bertrand | Zendy

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Development of a Luminex xTAG^®assay for cost-effective multiplex detection of β-lactamases in Gram-negative bacteria

Author(s) -

PieterJan Ceyssens,

Cristina Garcı́a-Graells,

Frédéric Fux,

Nadine Botteldoorn,

Wesley Mattheus,

Véronique Wuyts,

Sigrid C. J. De Keersmaecker,

Katelijne Dierick,

Sophie Bertrand

Publication year - 2016

Publication title -

journal of antimicrobial chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.124

H-Index - 194

eISSN - 1460-2091

pISSN - 0305-7453

DOI - 10.1093/jac/dkw201

Subject(s) - multiplex , genotyping , multiplex polymerase chain reaction , biology , microbiology and biotechnology , plasmid , escherichia coli , dna extraction , polymerase chain reaction , bacilli , genotype , gene , bacteria , genetics

The objective of this study was to design and validate a genotyping method for multiplex identification of ESBLs and carbapenemases in Gram-negative bacilli. This assay had to be (i) superior to traditional (multiplex) PCR/sequencing-based tests in turn-around time, gene coverage and the ability to detect multiple variants of the same allele, and (ii) significantly more cost-effective than commercial microarrays and WGS. The targeted β-lactamases include ESBLs (CTX-M families and subtypes, ESBL and non-ESBL SHV- and TEM-likes, OXA-1/2/7-likes, PER, VEB, GES), plasmid-mediated cephalosporinases (CMY, MOX, FOX, ACC, DHA, MIR/ACT) and carbapenemases (OXA-48, NDM, KPC, VIM, IMP).

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