Relative contribution of biological variation and technical variables to zone diameter variations of disc diffusion susceptibility testing | Zendy

Michael Hombach | Zendy; Carlos Ochoa | Zendy; Florian P. Maurer | Zendy; Tamara Pfiffner | Zendy; Erik C. Böttger | Zendy; Reinhard Furrer | Zendy

Open Access

Relative contribution of biological variation and technical variables to zone diameter variations of disc diffusion susceptibility testing

Author(s) -

Michael Hombach,

Carlos Ochoa,

Florian P. Maurer,

Tamara Pfiffner,

Erik C. Böttger,

Reinhard Furrer

Publication year - 2015

Publication title -

journal of antimicrobial chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.124

H-Index - 194

eISSN - 1460-2091

pISSN - 0305-7453

DOI - 10.1093/jac/dkv309

Subject(s) - diffusion , variation (astronomy) , statistics , total variation , enterococcus faecalis , biology , mathematics , variance (accounting) , physics , staphylococcus aureus , thermodynamics , bacteria , genetics , astrophysics , accounting , business

Disc diffusion is still largely based on manual procedures. Technical variations originate from inoculum preparation, variations in materials, individual operator plate streaking and reading accuracy. Resulting measurement imprecision contributes to categorization errors. Biological variation resembles the natural fluctuation of a measured parameter such as antibiotic susceptibility around a mean value. It is deemed to originate from factors such as genetic background or metabolic state. This study analysed the relative contribution of different technical and biological factors to total disc diffusion variation.

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