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Enterobacteriaceae clinical isolates showing amikacin resistance (MIC 64 to &gt;256 mg/L) in the absence of 16S rRNA methyltransferase (MTase) genes were found. The aim of this study was to clarify the molecular mechanisms underlying amikacin resistance in Enterobacteriaceae clinical isolates that do not produce 16S rRNA MTases.

New plasmid-mediated aminoglycoside 6′-N-acetyltransferase, AAC(6′)-Ian, and ESBL, TLA-3, from a Serratia marcescens clinical isolate