Development and evaluation of a novel universal β-lactamase gene subtyping assay for blaSHV, blaTEM and blaCTX-M using clinical and livestock-associated Escherichia coli | Zendy

Lena Strauß | Zendy; Carmen Dahms | Zendy; Karsten Becker | Zendy; Axel Krämer | Zendy; Martin Kaase | Zendy; Alexander Mellmann | Zendy

Open Access

Development and evaluation of a novel universal β-lactamase gene subtyping assay for blaSHV, blaTEM and blaCTX-M using clinical and livestock-associated Escherichia coli

Author(s) -

Lena Strauß,

Carmen Dahms,

Karsten Becker,

Axel Krämer,

Martin Kaase,

Alexander Mellmann

Publication year - 2014

Publication title -

journal of antimicrobial chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.124

H-Index - 194

eISSN - 1460-2091

pISSN - 0305-7453

DOI - 10.1093/jac/dku450

Subject(s) - subtyping , biology , amplicon , genetics , genotyping , escherichia coli , allele , typing , sanger sequencing , genotype , gene , polymerase chain reaction , molecular epidemiology , microbiology and biotechnology , computational biology , dna sequencing , computer science , programming language

Antibiotic resistance among Escherichia coli is globally an increasing problem in public healthcare. Understanding the spread of plasmid-mediated ESBL genes is of great importance in elucidating their molecular epidemiology. However, differentiation of subtypes and alleles is frequently hampered by the lack of comprehensive diagnostic tools. We therefore developed a novel universal blaSHV, blaTEM and blaCTX-M subtyping assay based on PCR and Sanger sequencing that results in large amplicons of >700 bp, enabling differentiation of bla alleles as precisely as possible.

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