Gene editing using a zinc-finger nuclease mimicking the CCR5Δ32 mutation induces resistance to CCR5-using HIV-1 | Zendy

Roger Badía | Zendy; Eva RiveiraMuñoz | Zendy; Bonaventura Clotet | Zendy; José A. Esté | Zendy; Ester Ballana | Zendy

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Gene editing using a zinc-finger nuclease mimicking the CCR5Δ32 mutation induces resistance to CCR5-using HIV-1

Author(s) -

Roger Badía,

Eva RiveiraMuñoz,

Bonaventura Clotet,

José A. Esté,

Ester Ballana

Publication year - 2014

Publication title -

journal of antimicrobial chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.124

H-Index - 194

eISSN - 1460-2091

pISSN - 0305-7453

DOI - 10.1093/jac/dku072

Subject(s) - zinc finger nuclease , biology , gene , genetics , in silico , frameshift mutation , virology , microbiology and biotechnology , mutation , zinc finger , transcription factor

To characterize a new zinc-finger nuclease (ZFN) that targets close to the sequence of the 32 bp deletion polymorphism in the CCR5 gene, and to generate cells resistant to HIV-1 strains that use CCR5. CCR5Δ32 is a naturally occurring deletion that provides genetic resistance to R5-tropic HIV-1. The specificity and efficacy of a newly identified target for CCR5 gene editing, near the CCR5Δ32 sequence (ZFNCCR5Δ32), was assessed as well as its ability to generate cells resistant to HIV infection with reduced off-target effects.

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