High-throughput informative single nucleotide polymorphism-based typing of Neisseria gonorrhoeae using the Sequenom MassARRAY iPLEX platform | Zendy

Ella Trembizki | Zendy; Helen Smith | Zendy; Monica M Lahra | Zendy; M. Chen | Zendy; Basil Donovan | Zendy; Christopher K. Fairley | Zendy; Rebecca Guy | Zendy; John Kaldor | Zendy; David G. Regan | Zendy; James Ward | Zendy; Michael D. Nissen | Zendy; Theo P. Sloots | Zendy; David M. Whiley | Zendy

Open Access

High-throughput informative single nucleotide polymorphism-based typing of Neisseria gonorrhoeae using the Sequenom MassARRAY iPLEX platform

Author(s) -

Ella Trembizki,

Helen Smith,

Monica M Lahra,

M. Chen,

Basil Donovan,

Christopher K. Fairley,

Rebecca Guy,

John Kaldor,

David G. Regan,

James Ward,

Michael D. Nissen,

Theo P. Sloots,

David M. Whiley

Publication year - 2014

Publication title -

journal of antimicrobial chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.124

H-Index - 194

eISSN - 1460-2091

pISSN - 0305-7453

DOI - 10.1093/jac/dkt544

Subject(s) - neisseria gonorrhoeae , typing , single nucleotide polymorphism , biology , genetics , neisseria , microbiology and biotechnology , genotype , gene , bacteria

Neisseria gonorrhoeae antimicrobial resistance (AMR) is a global problem heightened by emerging resistance to ceftriaxone. Appropriate molecular typing methods are important for understanding the emergence and spread of N. gonorrhoeae AMR. We report on the development, validation and testing of a Sequenom MassARRAY iPLEX method for multilocus sequence typing (MLST)-style genotyping of N. gonorrhoeae isolates.

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