Rapid single-colony whole-genome sequencing of bacterial pathogens | Zendy

Claudio U. Köser | Zendy; Louise Fraser | Zendy; Alexandros Ioannou | Zendy; Jennifer Becq | Zendy; M.J. Ellington | Zendy; Matthew T. G. Holden | Zendy; Sandra Reuter | Zendy; M. Estée Török | Zendy; Stephen D. Bentley | Zendy; Julian Parkhill | Zendy; N. A. Gormley | Zendy; Geoffrey Smith | Zendy; Sharon J. Peacock | Zendy

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Rapid single-colony whole-genome sequencing of bacterial pathogens

Author(s) -

Claudio U. Köser,

Louise Fraser,

Alexandros Ioannou,

Jennifer Becq,

M.J. Ellington,

Matthew T. G. Holden,

Sandra Reuter,

M. Estée Török,

Stephen D. Bentley,

Julian Parkhill,

N. A. Gormley,

Geoffrey Smith,

Sharon J. Peacock

Publication year - 2013

Publication title -

journal of antimicrobial chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.124

H-Index - 194

eISSN - 1460-2091

pISSN - 0305-7453

DOI - 10.1093/jac/dkt494

Subject(s) - neisseria meningitidis , biology , minion , whole genome sequencing , genome , dna sequencing , genotyping , microbiology and biotechnology , reference genome , bacterial genome size , pathogen , computational biology , genetics , gene , nanopore sequencing , bacteria , genotype

As a result of the introduction of rapid benchtop sequencers, the time required to subculture a bacterial pathogen to extract sufficient DNA for library preparation can now exceed the time to sequence said DNA. We have eliminated this rate-limiting step by developing a protocol to generate DNA libraries for whole-genome sequencing directly from single bacterial colonies grown on primary culture plates.

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