Multiplex PCR to detect the genes encoding naturally occurring oxacillinases in Acinetobacter spp. | Zendy

Witchuda Kamolvit | Zendy; Paul G. Higgins | Zendy; David L. Paterson | Zendy; Harald Seifert | Zendy

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Multiplex PCR to detect the genes encoding naturally occurring oxacillinases in Acinetobacter spp.

Author(s) -

Witchuda Kamolvit,

Paul G. Higgins,

David L. Paterson,

Harald Seifert

Publication year - 2013

Publication title -

journal of antimicrobial chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.124

H-Index - 194

eISSN - 1460-2091

pISSN - 0305-7453

DOI - 10.1093/jac/dkt480

Subject(s) - acinetobacter , acinetobacter calcoaceticus , biology , microbiology and biotechnology , multiplex polymerase chain reaction , amplicon , acinetobacter baumannii , neisseriaceae , polymerase chain reaction , gene , genetics , bacteria , antibiotics , pseudomonas aeruginosa

Bacteria of the genus Acinetobacter are increasingly being isolated in hospitals and are recognized as emerging nosocomial pathogens. Species identification is difficult and there is a need for simple molecular methods to differentiate between the species. Naturally occurring oxacillinase genes (blaOXA) have been identified in several Acinetobacter species and their detection by PCR can aid in species identification. The aim of this study was to develop a multiplex PCR to identify intrinsic blaOXA genes (i.e. bla(OXA-134-like), bla(OXA-211-like), bla(OXA-213-like), bla(OXA-214-like) and bla(OXA-228-like)) from Acinetobacter spp. for use as a tool for rapid species identification.

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