Harmonization of antimicrobial susceptibility testing by broth microdilution for Rhodococcus equi of animal origin
Author(s) -
Anne Riesenberg,
Andrea T. Feßler,
Cornelia Frömke,
Kristina Kadlec,
Dieter Klarmann,
Lothar Kreienbrock,
Christiane Werckenthin,
Štefan Schwarz
Publication year - 2013
Publication title -
journal of antimicrobial chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.124
H-Index - 194
eISSN - 1460-2091
pISSN - 0305-7453
DOI - 10.1093/jac/dkt134
Subject(s) - broth microdilution , rhodococcus equi , microbiology and biotechnology , antimicrobial , biology , minimum inhibitory concentration , biochemistry , virulence , gene
genes. The PCR-based replicon typing method showed that this blaNDM-1-positive plasmid belonged to the IncHI incompatibility group. Genetic structures surrounding the blaNDM-1 gene were investigated using a PCR mapping approach and sequencing. The blaNDM-1 gene was immediately bracketed by a truncated insertion sequence ISAba125 upstream and the bleomycin resistance gene bleMBL downstream. This genetic structure was similar to those observed surrounding the blaNDM-1 gene in Enterobacteriaceae. 7,10
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