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Validation of carbapenemase and extended-spectrum -lactamase multiplex endpoint PCR assays according to ISO 15189
Author(s) -
Pierre Bogaerts,
Roberta Rezende de Castro,
Ricardo De Mendonça,
TeDin Huang,
Olivier Denis,
Y. Glupczynski
Publication year - 2013
Publication title -
journal of antimicrobial chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.124
H-Index - 194
eISSN - 1460-2091
pISSN - 0305-7453
DOI - 10.1093/jac/dkt065
Subject(s) - multiplex polymerase chain reaction , multiplex , accreditation , amplicon , external quality assessment , broth microdilution , biology , medicine , microbiology and biotechnology , bioinformatics , polymerase chain reaction , gene , antibiotics , minimum inhibitory concentration , genetics , pathology , medical education
To validate and accredit a set of three multiplex endpoint PCR assays, targeting the most important carbapenemase and minor extended-spectrum β-lactamase (ESBL) resistance genes, according to the international ISO 15189 particular requirements for the quality and competence of medical laboratories.

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