Expression of SCCmec cassette chromosome recombinases in methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis | Zendy

Miloš Stojanov | Zendy; Olga Sakwińska | Zendy; Philippe Moreillon | Zendy

Open Access

Expression of SCCmec cassette chromosome recombinases in methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis

Author(s) -

Miloš Stojanov,

Olga Sakwińska,

Philippe Moreillon

Publication year - 2012

Publication title -

journal of antimicrobial chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.124

H-Index - 194

eISSN - 1460-2091

pISSN - 0305-7453

DOI - 10.1093/jac/dks494

Subject(s) - sccmec , recombinase , staphylococcus epidermidis , staphylococcus aureus , microbiology and biotechnology , biology , chromosome , gene cassette , horizontal gene transfer , mutant , methicillin resistant staphylococcus aureus , gene , antibiotic resistance , genetics , antibiotics , bacteria , genome , recombination , integron

Methicillin resistance in staphylococci is mediated by the mecA gene, which is carried on the staphylococcal cassette chromosome mec (SCCmec). SCCmec is responsible for vertical and horizontal transfer of methicillin resistance. Horizontal transfer implies first SCCmec excision from the chromosome. Site-specific excision is catalysed by the Ccr recombinases, which are encoded by ccrAB genes located on the cassette. The aim of this study is to determine the promoter activity of ccrAB genes in individual cells of methicillin-resistant Staphylococcus aureus (N315, COL and MW2) and Staphylococcus epidermidis (RP62A). One mutant cured of its SCCmec (N315EX) was also used. Exposure to various stresses was included in the study.

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