English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Sir, The major mechanism of resistance to macrolides, lincosamides and streptogramin B antibiotics (MLSB) is the methylation of the adenine at position A2058 in domain V of 23S rRNA. Of the rRNA methylase genes so far detected in staphylococci, erm(T) has rarely been identified. It was described for the first time in staphylococci on plasmid pKKS25 in a porcine livestockassociated methicillin-resistant Staphylococcus aureus (LA-MRSA) ST398 isolate. Recent studies also revealed its presence in the chromosomal DNA of methicillin-susceptible S. aureus (MSSA) ST398 isolates in humans. In this study, we investigated a previously identified erm(T)-positive MSSA ST398-t571 isolate from a healthy human to gain insight into the genetic environment of erm(T), its possible association with other resistance genes and its plasmid location. The MSSA ST398-t571 isolate C3912 was obtained in 2011 from the nasal swab of a healthy human. This isolate showed only inducible MLSB resistance. Plasmids were prepared and transformed into S. aureus RN4220 with subsequent selection on medium containing erythromycin (15 mg/L). A single plasmid, designated pUR3912, was identified and shown to confer the aforementioned resistance phenotype. Plasmid pUR3912 was linearized by EcoRI, cloned into the plasmid vector pBlueScript II SK+ (Stratagene) and the recombinant plasmid was transformed into Escherichia coli JM101. The complete plasmid pUR3912 was sequenced by primer walking on both strands starting with M13 universal and reverse primers. A schematic map of the 6182 bp plasmid pUR3912 is shown in Figure 1. The nucleotide sequence of plasmid pUR3912 determined in this study has been deposited in the EMBL database under accession number HE805623. The erm(T) gene encoded a 244 amino acid rRNA methylase which was indistinguishable from the recently described chromosomal Erm(T) of MSSA ST398 strain ST398NM01, and shared 98.9% identity (99.6% similarity) with Erm(T) of plasmid pKKS25 from LA-MRSA ST398. A comparison between the sequenced part of pKKS25 and pUR3912 revealed that the erm(T) gene was the only common feature between both plasmids (Figure 1a). A complete translational attenuator, which consisted of two pairs of inverted repeat sequences of 12 bp each and a reading frame for a regulatory peptide of 19 amino acids, was identified immediately upstream of the erm(T) gene (Figure 1b). Detailed analysis of the erm(T) region of pUR3912 showed that the erm(T) gene was flanked by two identical copies of the insertion sequence IS431 [named IS431L and IS431R based on their positions with respect to erm(T)], both located in the same orientation. A 709 bp region immediately downstream of IS431R showed 92.1% identity to the corresponding region of plasmid pSSP1 of Staphylococcus saprophyticus ATCC 15305 and included 120 bp of a truncated rep gene. A complete rep gene encoding a 204 amino acid replication initiation protein and a cadmium resistance operon were detected adjacent to this region. The rep gene showed 96.6% identity to that of pSSP1. The cadD gene encodes a 206 amino acid P-type metal efflux ATPase protein involved in cadmium resistance and cadX for a protein of 116 amino acids that serves as a transcriptional regulator of the cadmium resistance operon. Broth microdilution assays revealed that the S. aureus RN4220 transformant carrying pUR3912 exhibited a 128-fold increase in the MIC of CdSO4 (128 mg/L) as compared with S. aureus RN4220 (MIC CdSO4 1 mg/L), and thus confirmed the functionality of the cadDX operon. The comparison shown in Figure 1 suggested that a pUR3912-like plasmid has been incorporated into the chromosomal DNA of MSSA ST398NM01 and that insertion elements of the type IS431 were most likely involved in this process. Three structural differences were noted between pUR3912 and the pUR3912-like plasmid in the chromosomal DNA of MSSA ST398NM01 (Figure 1): (i) two direct repeats of 64 bp are present between cadX and rep in MSSA ST398NM01, while only one of these sequences is present in pUR3912; (ii) an additional IS431 copy, named IS431L, was present in pUR3912—the detection of the 8 bp direct repeats immediately upand downstream of the IS431L in pUR3912 strongly suggested that the integration of this insertion sequence into a pUR3912 precursor was an independent process; and (iii) the 709 bp pSSP1-like segment is missing in the chromosomally integrated plasmid, although the 9 bp (5′-TTTG-3′) corresponding to the 5′ terminus of the truncated rep gene are present. It is unknown when and by which way this 709 bp segment became part of pUR3912, but

Analysis of a novel erm(T)- and cadDX-carrying plasmid from methicillin-susceptible Staphylococcus aureus ST398-t571 of human origin