Intracellular forms of menadione-dependent small-colony variants of methicillin-resistant Staphylococcus aureus are hypersusceptible to -lactams in a THP-1 cell model due to cooperation between vacuolar acidic pH and oxidant species | Zendy

Laetitia G. Garcia | Zendy; Sandrine Lemaire | Zendy; Barbara C. Kahl | Zendy; Karsten Becker | Zendy; Richard A. Proctor | Zendy; Paul M. Tulkens | Zendy; Françoise Van Bambeke | Zendy

Open Access

Intracellular forms of menadione-dependent small-colony variants of methicillin-resistant Staphylococcus aureus are hypersusceptible to -lactams in a THP-1 cell model due to cooperation between vacuolar acidic pH and oxidant species

Author(s) -

Laetitia G. Garcia,

Sandrine Lemaire,

Barbara C. Kahl,

Karsten Becker,

Richard A. Proctor,

Paul M. Tulkens,

Françoise Van Bambeke

Publication year - 2012

Publication title -

journal of antimicrobial chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.124

H-Index - 194

eISSN - 1460-2091

pISSN - 0305-7453

DOI - 10.1093/jac/dks325

Subject(s) - microbiology and biotechnology , staphylococcus aureus , menadione , vancomycin , incubation , biology , antibacterial agent , mutant , chemistry , biochemistry , bacteria , antibiotics , genetics , gene , enzyme

Phagocytosed methicillin-resistant Staphylococcus aureus (MRSA) are susceptible to β-lactams because of an acid-induced conformational change of penicillin-binding protein (PBP) 2a within phagolysosomes. We have examined whether this mechanism applies to menD and hemB small-colony variants (SCVs) of the COL MRSA strain, using cloxacillin, meropenem, doripenem, and vancomycin as comparator.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research