Improved detection of genetic markers of antimicrobial resistance by hybridization probe-based melting curve analysis using primers to mask proximal mutations: examples include the influenza H275Y substitution | Zendy

David M. Whiley | Zendy; Kevin Jacob | Zendy; Jenny Nakos | Zendy; Cheryl Bletchly | Zendy; Graeme R. Nimmo | Zendy; Michael D. Nissen | Zendy; Theo P. Sloots | Zendy

Open Access

Improved detection of genetic markers of antimicrobial resistance by hybridization probe-based melting curve analysis using primers to mask proximal mutations: examples include the influenza H275Y substitution

Author(s) -

David M. Whiley,

Kevin Jacob,

Jenny Nakos,

Cheryl Bletchly,

Graeme R. Nimmo,

Michael D. Nissen,

Theo P. Sloots

Publication year - 2012

Publication title -

journal of antimicrobial chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.124

H-Index - 194

eISSN - 1460-2091

pISSN - 0305-7453

DOI - 10.1093/jac/dks040

Subject(s) - biology , typing , neisseria gonorrhoeae , melting curve analysis , nucleic acid thermodynamics , hybridization probe , virology , mutation , microbiology and biotechnology , genetics , dna , polymerase chain reaction , gene , base sequence

Numerous real-time PCR assays have been described for detection of the influenza A H275Y alteration. However, the performance of these methods can be undermined by sequence variation in the regions flanking the codon of interest. This is a problem encountered more broadly in microbial diagnostics.

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