Quantification of HIV-RNA from dried blood spots using the Siemens VERSANT(R) HIV-1 RNA (kPCR) assay | Zendy

Maria Franca Pirillo | Zendy; Patricia RecordonPinson | Zendy; Mauro Andreotti | Zendy; Maria Grazia Mancini | Zendy; Roberta Amici | Zendy; Marina Giuliano | Zendy

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Quantification of HIV-RNA from dried blood spots using the Siemens VERSANT(R) HIV-1 RNA (kPCR) assay

Author(s) -

Maria Franca Pirillo,

Patricia RecordonPinson,

Mauro Andreotti,

Maria Grazia Mancini,

Roberta Amici,

Marina Giuliano

Publication year - 2011

Publication title -

journal of antimicrobial chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.124

H-Index - 194

eISSN - 1460-2091

pISSN - 0305-7453

DOI - 10.1093/jac/dkr383

Subject(s) - dried blood , dried blood spot , viral load , rna , liter , spots , confidence interval , human immunodeficiency virus (hiv) , chromatography , medicine , biology , virology , chemistry , pathology , biochemistry , gene

Simplified methods for virological monitoring in resource-limited settings are increasingly needed. We evaluated the performance of the VERSANT(®) HIV-1 RNA (kPCR) assay for the determination of HIV-1 viral load from dried blood spots (DBS). Assay sensitivity and correlation with plasma quantification values were assessed.

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