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Rapid detection of resistance to pyrazinamide in Mycobacterium tuberculosis using the resazurin microtitre assay
Author(s) -
Paula Aline Zanetti Campanerut,
Luciana Dias Ghiraldi,
F.L.E. Spositto,
Daisy Nakamura Sato,
Clarice Q.F. Leite,
Mário Hiroyuki Hirata,
Rosário Dominguez Crespo Hirata,
Rosilene Fressatti Cardoso
Publication year - 2011
Publication title -
journal of antimicrobial chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.124
H-Index - 194
eISSN - 1460-2091
pISSN - 0305-7453
DOI - 10.1093/jac/dkr057
Subject(s) - resazurin , pyrazinamide , mycobacterium tuberculosis , tuberculosis , microbiology and biotechnology , broth microdilution , mycobacterium bovis , mycobacterium , medicine , chromatography , biology , chemistry , minimum inhibitory concentration , antibiotics , pathology
The resazurin microtitre plate assay (REMA) was evaluated to determine the susceptibility of Mycobacterium tuberculosis to pyrazinamide, and was compared with the broth microdilution method (BMM), the absolute concentration method (ACM) and pyrazinamidase (PZase) determination.

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