Evaluation of a rapid detection method of clarithromycin resistance genes in Mycobacterium avium complex isolates | Zendy

Takayuki Inagaki | Zendy; Tetsuya Yagi | Zendy; Kazuya Ichikawa | Zendy; Taku Nakagawa | Zendy; Makoto Moriyama | Zendy; Keiichi Uchiya | Zendy; Toshiaki Nikai | Zendy; Kazuma Ogawa | Zendy

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Evaluation of a rapid detection method of clarithromycin resistance genes in Mycobacterium avium complex isolates

Author(s) -

Takayuki Inagaki,

Tetsuya Yagi,

Kazuya Ichikawa,

Taku Nakagawa,

Makoto Moriyama,

Keiichi Uchiya,

Toshiaki Nikai,

Kazuma Ogawa

Publication year - 2011

Publication title -

journal of antimicrobial chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.124

H-Index - 194

eISSN - 1460-2091

pISSN - 0305-7453

DOI - 10.1093/jac/dkq536

Subject(s) - 23s ribosomal rna , clarithromycin , biology , microbiology and biotechnology , drug resistance , broth microdilution , ribosomal rna , gene , minimum inhibitory concentration , antibiotics , genetics , rna , ribosome

Clarithromycin is the key drug in the various treatment regimens of Mycobacterium avium complex (MAC) diseases, and is the only drug for which drug susceptibility has been shown to correlate with clinical response in these diseases. A point mutation at either positions 2058 or 2059 of the 23S rRNA gene has been reported to occur in high-level clarithromycin-resistant isolates. In this study, we examined the correlation between the results from a drug susceptibility test and the mutation of the 23S rRNA gene involved in drug resistance in MAC. Furthermore, we adapted a rapid detection method using amplification refractory mutation system (ARMS)-PCR to identify mutations in the 23S rRNA gene in MAC isolates.

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