A 96-well plate fluorescence assay for assessment of cellular permeability and active efflux in Salmonella enterica serovar Typhimurium and Escherichia coli | Zendy

N. G. Coldham | Zendy; Mark Webber | Zendy; Martin J. Woodward | Zendy; Laura J. V. Piddock | Zendy

Open Access

A 96-well plate fluorescence assay for assessment of cellular permeability and active efflux in Salmonella enterica serovar Typhimurium and Escherichia coli

Author(s) -

N. G. Coldham,

Mark Webber,

Martin J. Woodward,

Laura J. V. Piddock

Publication year - 2010

Publication title -

journal of antimicrobial chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.124

H-Index - 194

eISSN - 1460-2091

pISSN - 0305-7453

DOI - 10.1093/jac/dkq169

Subject(s) - salmonella enterica , microbiology and biotechnology , escherichia coli , efflux , serotype , salmonella , enterobacteriaceae , bacteria , biology , chemistry , biochemistry , genetics , gene

Multiply antibiotic-resistant (MAR) mutants of Escherichia coli and Salmonella enterica are characterized by reduced susceptibility to several unrelated antibiotics, biocides and other xenobiotics. Porin loss and/or active efflux have been identified as a key mechanisms of MAR. A single rapid test was developed for MAR.

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