Quantitative multiplex real-time PCR for detecting class 1, 2 and 3 integrons | Zendy

Olivier Barraud | Zendy; M.C. Baclet | Zendy; F. Denis | Zendy; Marie-Cécile Ploy | Zendy

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Quantitative multiplex real-time PCR for detecting class 1, 2 and 3 integrons

Author(s) -

Olivier Barraud,

M.C. Baclet,

F. Denis,

Marie-Cécile Ploy

Publication year - 2010

Publication title -

journal of antimicrobial chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.124

H-Index - 194

eISSN - 1460-2091

pISSN - 0305-7453

DOI - 10.1093/jac/dkq167

Subject(s) - integron , taqman , biology , primer (cosmetics) , multiplex , polymerase chain reaction , real time polymerase chain reaction , microbiology and biotechnology , bacteria , gene , genetics , antibiotic resistance , chemistry , organic chemistry

Integrons are bacterial genetic elements that can capture and express genes contained in mobile cassettes. Integrons have been described worldwide in Gram-negative bacteria and are a marker of antibiotic resistance. We developed a specific and sensitive Taqman probe-based real-time PCR method with three different primer-probe pairs for simultaneous detection of the three main classes of integron.

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