A novel DNA gyrase inhibitor rescues Escherichia coli dnaAcos mutant cells from lethal hyperinitiation | Zendy

Lars Gunnar Johnsen | Zendy; Christoph Weigel | Zendy; Jens Peter von Kries | Zendy; Mona Møller | Zendy; Kirsten Skarstad | Zendy

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A novel DNA gyrase inhibitor rescues Escherichia coli dnaAcos mutant cells from lethal hyperinitiation

Author(s) -

Lars Gunnar Johnsen,

Christoph Weigel,

Jens Peter von Kries,

Mona Møller,

Kirsten Skarstad

Publication year - 2010

Publication title -

journal of antimicrobial chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.124

H-Index - 194

eISSN - 1460-2091

pISSN - 0305-7453

DOI - 10.1093/jac/dkq071

Subject(s) - dna gyrase , dnaa , escherichia coli , dna replication , novobiocin , mutant , dna , biology , in vitro , biochemistry , chemistry , microbiology and biotechnology , antibiotics , origin of replication , gene

In order to search for novel antibacterial compounds we used a previously developed screening strain designed specifically to discover inhibitors of the bacterial initiator protein, DnaA. This strain (SF53) is not viable at 30 degrees C due to overinitiation. Therefore, compounds that are able to restore growth to SF53 cells are likely to cause either partial or complete inhibition of DnaA function. In this study we used SF53 cells to screen the Library of Pharmacologically Active Compounds (LOPAC).

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