Determination of the susceptibility of Mycobacterium tuberculosis to pyrazinamide in liquid and solid media assessed by a colorimetric nitrate reductase assay | Zendy

Heidi Syre | Zendy; Kristi Øvreås | Zendy; Harleen M. S. Grewal | Zendy

Open Access

Determination of the susceptibility of Mycobacterium tuberculosis to pyrazinamide in liquid and solid media assessed by a colorimetric nitrate reductase assay

Author(s) -

Heidi Syre,

Kristi Øvreås,

Harleen M. S. Grewal

Publication year - 2010

Publication title -

journal of antimicrobial chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.124

H-Index - 194

eISSN - 1460-2091

pISSN - 0305-7453

DOI - 10.1093/jac/dkp506

Subject(s) - pyrazinamide , microbiology and biotechnology , mycobacterium tuberculosis , tuberculosis , nitrate reductase , chemistry , biology , medicine , biochemistry , enzyme , pathology

The aim of this study was to develop and evaluate two colorimetric nitrate reductase-based antibiotic susceptibility (CONRAS) tests, the CONRAS-liquid test and the CONRAS-LJ test, to enable susceptibility testing of Mycobacterium tuberculosis to pyrazinamide. To enhance the growth of M. tuberculosis in 7H9 broth with acid pH (6.0), the effect of three potential growth-promoting substances (reconstitution fluid, fastidious organism supplement and epidermal growth factor) was evaluated.

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