Characterization of fluoroquinolone resistance in a clinical isolate of Pseudomonas stutzeri | Zendy

José Manuel Rodríguez-Martínez | Zendy; Laurent Poirel | Zendy; Nashwan al Naiemi | Zendy; Y. J. Debets-Ossenkopp | Zendy; Patrice Nordmann | Zendy

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Characterization of fluoroquinolone resistance in a clinical isolate of Pseudomonas stutzeri

Author(s) -

José Manuel Rodríguez-Martínez,

Laurent Poirel,

Nashwan al Naiemi,

Y. J. Debets-Ossenkopp,

Patrice Nordmann

Publication year - 2009

Publication title -

journal of antimicrobial chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.124

H-Index - 194

eISSN - 1460-2091

pISSN - 0305-7453

DOI - 10.1093/jac/dkp441

Subject(s) - pseudomonas stutzeri , amplicon , microbiology and biotechnology , quinolone , biology , polymerase chain reaction , gene , chemistry , bacteria , genetics , antibiotics

common mechanisms of fluoroquinolone resistance in Gramnegative organisms, whole-cell DNA of isolate 13 was extracted and used as a template for PCR amplification using primers able to amplify the corresponding quinolone resistance determining regions (‘QRDRs’) of the gyrA and parC genes of P. stutzeri ATCC 17588 (as control) and of P. stutzeri 13, and subsequently sequenced. The primers used were: GyrA-PstF (5 0 -CAT GGG CGA ACT GGC CAA AG-3 0 ) and GyrA-PstR (5 0 -CTG CCG GAA TCT GCT CGG TG-3 0 ) for gyrA (expected amplicon of 473 bp); and ParC-PstF (5 0 -ATG AGC GAA TCC CTC GAC CTG-3 0 ) and ParC-PstR (5 0 -GGT TCG TCC AGG GTG CCG TCG A-3 0 ) for parC (expected amplicon of

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