Rapid identification of multidrug-resistant Mycobacterium tuberculosis isolates by rpoB gene scanning using high-resolution melting curve PCR analysis | Zendy

Ariane Pietzka | Zendy; A. Indra | Zendy; A. Stoger | Zendy; J. Zeinzinger | Zendy; M. Konrad | Zendy; P. Hasenberger | Zendy; Franz Allerberger | Zendy; Werner Ruppitsch | Zendy

Open Access

Rapid identification of multidrug-resistant Mycobacterium tuberculosis isolates by rpoB gene scanning using high-resolution melting curve PCR analysis

Author(s) -

Ariane Pietzka,

A. Indra,

A. Stoger,

J. Zeinzinger,

M. Konrad,

P. Hasenberger,

Franz Allerberger,

Werner Ruppitsch

Publication year - 2009

Publication title -

journal of antimicrobial chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.124

H-Index - 194

eISSN - 1460-2091

pISSN - 0305-7453

DOI - 10.1093/jac/dkp124

Subject(s) - rpob , high resolution melt , rifampicin , melting curve analysis , mycobacterium tuberculosis , tuberculosis , multiple drug resistance , biology , drug resistance , microbiology and biotechnology , virology , genotype , polymerase chain reaction , medicine , gene , antibiotics , genetics , pathology

Multidrug-resistant (MDR) Mycobacterium tuberculosis poses a serious threat to the control of tuberculosis (TB) and constitutes an increasing public health problem. The availability of rapid in vitro susceptibility tests is a prerequisite for optimal patient treatment. Rifampicin resistance caused by diverse mutations in the rpoB gene is an established and widely used surrogate marker for MDR-TB. We used a high-resolution melting (HRM) curve analysis approach to scan for mutations in the rpoB gene.

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