Application of denaturing HPLC to rapidly identify rifampicin-resistant Mycobacterium tuberculosis in low- and high-prevalence areas | Zendy

Jason T. Evans | Zendy; Asia Parveen | Zendy; Glenn E. Smith | Zendy; Li Xu | Zendy; Ella Wai-Ching Chan | Zendy; Raphael C. Y. Chan | Zendy; Peter M. Hawkey | Zendy

Open Access

Application of denaturing HPLC to rapidly identify rifampicin-resistant Mycobacterium tuberculosis in low- and high-prevalence areas

Author(s) -

Jason T. Evans,

Asia Parveen,

Glenn E. Smith,

Li Xu,

Ella Wai-Ching Chan,

Raphael C. Y. Chan,

Peter M. Hawkey

Publication year - 2008

Publication title -

journal of antimicrobial chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.124

H-Index - 194

eISSN - 1460-2091

pISSN - 0305-7453

DOI - 10.1093/jac/dkn506

Subject(s) - rifampicin , mycobacterium tuberculosis , microbiology and biotechnology , tuberculosis , biology , medicine , antibiotics , virology , pathology

Since the emergence of multidrug-resistant and extensively drug-resistant tuberculosis, there has been a call for a rapid assay to detect rifampicin-resistant strains that can be implemented into a routine service to analyse all strains in a specific geographical location. Denaturing HPLC (dHPLC) is a rapid screening test that can detect mutations in PCR amplicons. The aim of this study was to evaluate the dHPLC analysis of rifampicin-resistant Mycobacterium tuberculosis isolates using an extensive strain collection from Hong Kong and the UK and a collection of 84 consecutive clinical isolates.

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