Functional characterization of Tn1331 gene cassettes | Zendy

María Soledad Ramirez | Zendy; T Richard Parenteau | Zendy; Daniela Centrón | Zendy; Marcelo E. Tolmasky | Zendy

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Functional characterization of Tn1331 gene cassettes

Author(s) -

María Soledad Ramirez,

T Richard Parenteau,

Daniela Centrón,

Marcelo E. Tolmasky

Publication year - 2008

Publication title -

journal of antimicrobial chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.124

H-Index - 194

eISSN - 1460-2091

pISSN - 0305-7453

DOI - 10.1093/jac/dkn279

Subject(s) - biology , transposable element , plasmid , integrase , gene , microbiology and biotechnology , dna , escherichia coli , gene cassette , genetics , transposase , recombinant dna , integron , dna sequencing , mutant

The transposon Tn1331 possesses a region including three antibiotic resistance genes with the structure aac(6')-Ib-attC-aadA1-attI1*-bla(OXA-9)-attC, which potentially includes four gene cassettes. Experimental data on the mobility of fusion cassettes as well as those on mobility of cassettes in a genetic environment such as Tn1331, which lacks an integrase gene, are limited. Therefore, experiments using pJHCMW1, a plasmid harbouring this transposon, in the presence of IntI1 supplied in trans were carried out to define which cassettes are mobile in vivo.

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