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description. To our knowledge, this is also the first report of group 2 CTX-M enzymes in the UK; the isolates were from three hospitals and it is possible that they represent separate introductions. Group 2 are the most prevalent CTX-M enzymes in parts of South America and in Israel; group 9 enzymes are prevalent in Spain. In summary, we have developed a multiplex PCR assay that was able to detect and distinguish alleles encoding CTX-M enzymes belonging to all five phylogenetic groups. CTX-M ESBLs are recognized worldwide as an increasingly serious public health concern. Simple PCR assays, as described here and by others, will assist in monitoring their emergence and dissemination.

Development of highly ciprofloxacin-resistant laboratory mutants of Acinetobacter baumannii lacking topoisomerase IV gene mutations