Antisense phosphorodiamidate morpholino oligomer inhibits viability of Escherichia coli in pure culture and in mouse peritonitis | Zendy

Bruce L. Geller | Zendy; Jesse D. Deere | Zendy; Lucas D. Tilley | Zendy; Patrick L. Iversen | Zendy

Open Access

Antisense phosphorodiamidate morpholino oligomer inhibits viability of Escherichia coli in pure culture and in mouse peritonitis

Author(s) -

Bruce L. Geller,

Jesse D. Deere,

Lucas D. Tilley,

Patrick L. Iversen

Publication year - 2005

Publication title -

journal of antimicrobial chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.124

H-Index - 194

eISSN - 1460-2091

pISSN - 0305-7453

DOI - 10.1093/jac/dki129

Subject(s) - escherichia coli , morpholino , cell culture , biology , microbiology and biotechnology , oligomer , chemistry , gene , biochemistry , gene knockdown , genetics , organic chemistry

Antisense phosphorodiamidate morpholino oligomers (PMOs) are synthetic DNA mimics that specifically inhibit gene expression in pure cultures of Escherichia coli. Previously, an 11 base PMO targeted to an essential gene (acpP) for phospholipid biosynthesis was shown to inhibit growth of a pure culture of E. coli AS19, which has an abnormally permeable outer membrane. The objectives of experiments in this report are to show that the AcpP PMO significantly inhibits growth of strain SM105, which has a normal, intact outer membrane, both in pure culture and in infected mice.

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