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Calcium-supplemented daptomycin Etest strips for susceptibility testing on Iso-Sensitest agar
Author(s) -
Alan P. Johnson
Publication year - 2004
Publication title -
journal of antimicrobial chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.124
H-Index - 194
eISSN - 1460-2091
pISSN - 0305-7453
DOI - 10.1093/jac/dkh198
Subject(s) - daptomycin , etest , agar , calcium , medicine , chemistry , microbiology and biotechnology , antibiotics , biology , vancomycin , staphylococcus aureus , bacteria , genetics
Iso-Sensitest agar (ISA), which is recommended by the BSAC for routine susceptibility testing of staphylococci and enterococci, contains insufficient calcium for testing daptomycin. Isotonic agar supplemented with 50 mg/L calcium has been advocated, but is not routinely available in many laboratories. We evaluated a daptomycin Etest that incorporates a constant level of calcium throughout the daptomycin gradient, designed to give an appropriate concentration around the strip during testing, as an alternative for susceptibility testing on ISA.

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