A multidrug efflux phenotype mutant of Streptococcus pyogenes | Zendy

H. E. Jones | Zendy

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A multidrug efflux phenotype mutant of Streptococcus pyogenes

Author(s) -

H. E. Jones

Publication year - 2003

Publication title -

journal of antimicrobial chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.124

H-Index - 194

eISSN - 1460-2091

pISSN - 0305-7453

DOI - 10.1093/jac/dkg121

Subject(s) - ethidium bromide , acriflavine , norfloxacin , efflux , mutant , microbiology and biotechnology , chemistry , streptococcus pyogenes , multiple drug resistance , biology , biochemistry , antibiotics , bacteria , ciprofloxacin , dna , staphylococcus aureus , genetics , gene

We describe a mutant of Streptococcus pyogenes NCTC 8198 with a multidrug efflux phenotype. A mutant selected with ethidium bromide showed a four-fold rise in MIC of norfloxacin, a 16-fold rise in MIC of ethidium bromide and an eight-fold rise in MIC of acriflavine when compared with the parent strain. The MICs were unaffected by the efflux pump inhibitors reserpine, rescinnamine and verapamil. The mutant's ethidium bromide MIC was reduced two-fold by norfloxacin. Ethidium bromide accumulation after 10 min was 58% lower in the mutant compared with the parent. This difference was not affected by carbonyl cyanide m-chlorophenylhydrazone.

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