Application of a spectrophotometric method for the determination of post-antibiotic effect and comparison with viable counts in agar | Zendy

M C Domínguez | Zendy; Matteo de Rosa | Zendy; M V Borobio | Zendy

Open Access

Application of a spectrophotometric method for the determination of post-antibiotic effect and comparison with viable counts in agar

Author(s) -

M C Domínguez,

Matteo de Rosa,

M V Borobio

Publication year - 2001

Publication title -

journal of antimicrobial chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.124

H-Index - 194

eISSN - 1460-2091

pISSN - 0305-7453

DOI - 10.1093/jac/47.4.391

Subject(s) - gentamicin , staphylococcus aureus , ciprofloxacin , agar , spectrophotometry , pseudomonas aeruginosa , escherichia coli , chromatography , microbiology and biotechnology , pseudomonas , chemistry , antibiotics , biology , bacteria , biochemistry , genetics , gene

The post-antibiotic effects of gentamicin and ciprofloxacin at 1x, 2x and 4x MIC on Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 29213 were studied using a spectrophotometric method and the classic method of viable counts on agar as a reference. Monitoring of the growth kinetics was carried out by viability counting on the plate every hour and by means of the optical density of the cultures measured by spectrophotometry at a wavelength of 450 nm. No statistically significant differences were found between the results obtained with the spectrophotometric method and the reference method. The former method was much quicker, much easier to use and to replicate.

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