The effect of fosfomycin on neutrophil function | Zendy

Robert Krause | Zendy

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The effect of fosfomycin on neutrophil function

Author(s) -

Robert Krause

Publication year - 2001

Publication title -

journal of antimicrobial chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.124

H-Index - 194

eISSN - 1460-2091

pISSN - 0305-7453

DOI - 10.1093/jac/47.2.141

Subject(s) - fosfomycin , chemokinesis , phagocytosis , flow cytometry , intracellular , extracellular , incubation , calcium in biology , microbiology and biotechnology , cytosol , biology , granulocyte , chemistry , biochemistry , chemotaxis , immunology , antibiotics , enzyme , receptor

Fosfomycin (cis-1,2-epoxypropyl phosphonic acid) is a cell wall synthesis-inhibiting antibiotic. We investigated the effect of fosfomycin on several indices of neutrophil function. Neutrophil phagocytosis was analysed by flow cytometry. Cytosolic calcium kinetics were assessed fluorometrically and neutrophil bactericidal ability was assessed by fluorescence microscopy. Intracellular reactive oxygen intermediate (ROI) production was analysed by flow cytometry and extracellular ROI by cytochrome c reductase assay. After fosfomycin incubation, phagocytosis was unaffected as assessed by the FACS assay. Fosfomycin incubation resulted in enhanced bactericidal ability, in increased intracellular calcium concentrations, elevated extracellular ROI production and decreased chemotaxis but it did not affect intracellular ROI production and chemokinesis.

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