In vitro selected fluoroquinolone-resistant mutants of Citrobacter freundii: analysis of the quinolone resistance acquisition | Zendy

María M. Tavío | Zendy; Jordi Vilà | Zendy; Joaquı́m Ruiz | Zendy; Gianfranco Amicosante | Zendy; Nicola Franceschini | Zendy; Antonio Manuel MartínSánchez | Zendy; M. T. De Jiménez Anta | Zendy

Open Access

In vitro selected fluoroquinolone-resistant mutants of Citrobacter freundii: analysis of the quinolone resistance acquisition

Author(s) -

María M. Tavío,

Jordi Vilà,

Joaquı́m Ruiz,

Gianfranco Amicosante,

Nicola Franceschini,

Antonio Manuel MartínSánchez,

M. T. De Jiménez Anta

Publication year - 2000

Publication title -

journal of antimicrobial chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.124

H-Index - 194

eISSN - 1460-2091

pISSN - 0305-7453

DOI - 10.1093/jac/45.4.521

Subject(s) - citrobacter freundii , quinolone , mutant , efflux , microbiology and biotechnology , in vitro , gatifloxacin , dna gyrase , citrobacter , biology , enterobacteriaceae , chemistry , escherichia coli , antibiotics , levofloxacin , gene , biochemistry

Ten quinolone-resistant mutants of Citrobacter freundii, which were selected in vitro with fluoroquinolones from two clinical isolates, were studied. The parent isolates were susceptible to quinolones in spite of showing a single substitution in the GyrB (His-417 --> Leu). No change was observed in the outer membrane proteins or in the lipopolysaccharide in any of the ten mutants studied with respect to their parent isolates. The development of quinolone resistance in selected mutants was associated with the appearance of a substitution in the GyrA (Thr-83 --> Ile) in nine of the ten mutants plus enhanced active efflux in all of them.

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