Inoculum preparation for in-vitro susceptibility testing of filamentous fungi.
Author(s) -
Josep Guarro,
Isabel Pujol,
Carme Aguilar,
Carles Llop,
Joan FernándezBallart
Publication year - 1998
Publication title -
journal of antimicrobial chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.124
H-Index - 194
eISSN - 1460-2091
pISSN - 0305-7453
DOI - 10.1093/jac/42.3.385
Subject(s) - conidium , hemocytometer , biology , microbiology and biotechnology , in vitro , propagule , botany , biochemistry
The percent transmission (T) and the equivalence in cfu/mL of two suspensions of 125 isolates of clinically important filamentous fungi containing 1-5 x 10(5) and 1-5 x 10(4) conidia/mL, adjusted with a haemocytometer, were determined. A wide variation of T values was observed, especially with the first inoculum. A good agreement was obtained between the counted and the viable propagules. The haemocytometric method is considered a useful technique for standardizing inocula.
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