Application of X-ray micrography and imaging to study the effect of gentamicin on Pseudomonas aeruginosa | Zendy

Jayshree Rajyaguru | Zendy

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Application of X-ray micrography and imaging to study the effect of gentamicin on Pseudomonas aeruginosa

Author(s) -

Jayshree Rajyaguru

Publication year - 1998

Publication title -

journal of antimicrobial chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.124

H-Index - 194

eISSN - 1460-2091

pISSN - 0305-7453

DOI - 10.1093/jac/41.5.557

Subject(s) - gentamicin , micrography , pseudomonas aeruginosa , membrane , biophysics , microbiology and biotechnology , cell membrane , intracellular , chemistry , materials science , antibiotics , biology , bacteria , biochemistry , scanning electron microscope , genetics , composite material

Aminoglycosides disrupt the outer membrane of Pseudomonas aeruginosa to facilitate access to their intracellular target. High-resolution X-ray micrography of live specimens is a relatively new technique. We used laser (nanosecond) plasma to image live cells of P. aeruginosa ATCC 27853. After exposure to 25 mg/L gentamicin for 15 min. we observed perturbation of the cell surface, membrane blebbings (370 nm and 273 nm diameter) away from the cell, formation of distinct channels (241 nm long) resulting from indentation and induction of cell elongation from 3-3.6 microm (control) to 4.6-5.26 microm (gentamicin-treated cells). These data illustrate the potential of high-resolution X-ray micrography for studying effects of drugs on live microbiological specimens.

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