PCR single strand conformational polymorphism can be used to detect the gene encoding SHV-7 extended-spectrum beta-lactamase and to identify different SHV genes within the same strain | Zendy

Fatima M’Zali | Zendy; John Heritage | Zendy; Deborah GascoyneBinzi | Zendy; A.M. Snelling | Zendy; Peter M. Hawkey | Zendy

Open Access

PCR single strand conformational polymorphism can be used to detect the gene encoding SHV-7 extended-spectrum beta-lactamase and to identify different SHV genes within the same strain

Author(s) -

Fatima M’Zali,

John Heritage,

Deborah GascoyneBinzi,

A.M. Snelling,

Peter M. Hawkey

Publication year - 1998

Publication title -

journal of antimicrobial chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.124

H-Index - 194

eISSN - 1460-2091

pISSN - 0305-7453

DOI - 10.1093/jac/41.1.123

Subject(s) - klebsiella pneumoniae , single strand conformation polymorphism , gene , biology , genetics , beta lactamase , strain (injury) , mutant , polymerase chain reaction , microbiology and biotechnology , escherichia coli , anatomy

The PCR single strand conformational polymorphism (PCR-SSCP) technique described to identify mutants of the SHV beta-lactamases was extended to identify an SHV-7 type beta-lactamase. This was found in a strain of Klebsiella pneumoniae, the first recorded isolate in the UK to produce this type of enzyme. We also demonstrate that PCR-SSCP can be used to identify more than one SHV beta-lactamase gene in a single strain.

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