Manipulation of immune system via immortal bone marrow stem cells | Zendy

Christiane Ruedl | Zendy; Hanif Javanmard Khameneh | Zendy; K Karjalainen | Zendy

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Manipulation of immune system via immortal bone marrow stem cells

Author(s) -

Christiane Ruedl,

Hanif Javanmard Khameneh,

K Karjalainen

Publication year - 2008

Publication title -

international immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.86

H-Index - 134

eISSN - 1460-2377

pISSN - 0953-8178

DOI - 10.1093/intimm/dxn079

Subject(s) - stem cell , haematopoiesis , bone marrow , microbiology and biotechnology , biology , progenitor cell , multipotent stem cell , in vitro , immune system , clinical uses of mesenchymal stem cells , immunology , cancer research , adult stem cell , endothelial stem cell , genetics

Extensive amplification of hematopoietic stem cells (HSCs) and their multipotent primitive progenitors (MPPs) in culture would greatly benefit not only clinical transplantation but also provide a potential tool to manipulate all cellular lineages derived from these cells for gene therapy and experimental purposes. Here, we demonstrate that mouse bone marrow cultures containing cells engineered to over-express NUP98-HOXB4 fusion protein support self-renewal of physiologically normal HSC and MPP for several weeks leading practically to their unlimited expansion. This allows time consuming and cumulative in vitro experimental manipulations without sacrificing their ability to differentiate in vivo or in vitro to any hematopoietic lineage.

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