Open AccessAltered CD45 isoform expression affects lymphocyte function in CD45 Tg mice
Author(s) -
Elma Tchilian,
Ritu Dawes,
Lisa Hyland,
María Montoya,
Agnès Le Bon,
Persephone Borrow,
Sam Hou,
David F. Tough,
Peter C. L. Beverley
Publication year - 2004
Publication title -
international immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.86
H-Index - 134
eISSN - 1460-2377
pISSN - 0953-8178
DOI - 10.1093/intimm/dxh135
Subject(s) - gene isoform , biology , phenotype , genetically modified mouse , cd8 , transgene , t cell , microbiology and biotechnology , immune system , lymphocytic choriomeningitis , immunology , gene , genetics
Transgenic mice have been constructed expressing high (CD45RABC) and low (CD45R0) molecular weight CD45 isoforms on a CD45-/- background. Phenotypic analysis and in vivo challenge of these mice with influenza and lymphocytic choriomeningitis viruses shows that T cell differentiation and peripheral T cell function are related to the level of CD45 expression but not to which CD45 isoform is expressed. In contrast, B cell differentiation is not restored, irrespective of the level of expression of a single isoform. All CD45 trangenic mice have T cells with an activated phenotype and increased T cell turnover. These effects are more prominent in CD8 than CD4 cells. The transgenic mice share several properties with humans expressing variant CD45 alleles and provide a model to understand immune function in variant individuals.