Th2-specific DNase I-hypersensitive sites in the murine IL-13 and IL-4 intergenic region. | Zendy

Naofumi Takemoto | Zendy; Naoko KoyanoNakagawa | Zendy; Toyokazu Yokota | Zendy; Naoko Arai | Zendy; Shoichiro Miyatake | Zendy; Kenichi Arai | Zendy

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Th2-specific DNase I-hypersensitive sites in the murine IL-13 and IL-4 intergenic region.

Author(s) -

Naofumi Takemoto,

Naoko KoyanoNakagawa,

Toyokazu Yokota,

Naoko Arai,

Shoichiro Miyatake,

Kenichi Arai

Publication year - 1998

Publication title -

international immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.86

H-Index - 134

eISSN - 1460-2377

pISSN - 0953-8178

DOI - 10.1093/intimm/10.12.1981

Subject(s) - hypersensitive site , biology , locus (genetics) , gene , microbiology and biotechnology , dnase i hypersensitive site , t cell receptor , effector , transgene , intergenic region , genetics , t cell , promoter , gene expression , genome , immune system

IL-4 and IL-13 are cytokines preferentially produced by Th2 cells, and their genes are located in close proximity on human chromosome 5 and mouse chromosome 11. To identify potential regulatory elements that confer Th2-specific expression of IL-4 and IL-13 genes, we constructed a physical map of the IL-13/IL-4 locus and conducted DNase I-hypersensitive (DH) site analysis using Th clones and in vitro-differentiated effector Th cells obtained from TCR transgenic mice. Three DH sites, HSS1, HSS2 and HSS3, were identified within the intergenic region between IL-13 and IL-4 genes. HSS3 was observed both in Th1 and Th2 cells as well as CD4+ naive T cells, while HSS1 and HSS2 were detected exclusively in Th2 cells. The correlation between differentiation into Th2 subtype and the appearance of HSS1 and HSS2 suggests that these regions may play a role in subtype-specific expression of the IL-13/IL-4 locus.

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