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Toxin A has historically been regarded as the primary virulence determinant in Clostridium difficile infection, but naturally occurring toxin A-negative, toxin B-positive (A-/B+) C. difficile strains are known to be virulent. To determine the role of toxin B in these strains, we immunized hamsters with a toxoid prepared from purified toxin B to determine whether they would be protected from lethal challenge with an A-/B+ strain of C. difficile.

the journal of infectious diseases (online. university of chicago press)/the journal of infectious diseases

Vaccination With Parenteral Toxoid B Protects Hamsters Against Lethal Challenge With Toxin A–Negative, Toxin B–Positive Clostridium difficile but Does Not Prevent Colonization