Open AccessHuman Respiratory Syncytial Virus-Induced Immune Signature of Infection Revealed by Transcriptome Analysis of Clinical Pediatric Nasopharyngeal Swab Samples
Author(s) -
Claire Nicolas de Lamballerie,
Andrés Pizzorno,
Julia Dubois,
Blandine Padey,
Thomas Julien,
Aurélien Traversier,
Julie Carbonneau,
Elody Orcel,
Bruno Lina,
MarieÈve Hamelin,
Magali Roche,
Julien Textoris,
Guy Boivin,
Catherine Legras-Lachuer,
Benjamin Terrier,
Manuel RosaCalatrava
Publication year - 2020
Publication title -
the journal of infectious diseases (online. university of chicago press)/the journal of infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.69
H-Index - 252
eISSN - 1537-6613
pISSN - 0022-1899
DOI - 10.1093/infdis/jiaa468
Subject(s) - transcriptome , virus , immune system , virology , immunology , biology , respiratory system , medicine , gene , gene expression , genetics , anatomy
Human respiratory syncytial virus (HRSV) constitutes one the main causes of respiratory infection in neonates and infants worldwide. Transcriptome analysis of clinical samples using high-throughput technologies remains an important tool to better understand virus-host complex interactions in the real-life setting but also to identify new diagnosis/prognosis markers or therapeutics targets. A major challenge when exploiting clinical samples such as nasal swabs, washes, or bronchoalveolar lavages is the poor quantity and integrity of nucleic acids. In this study, we applied a tailored transcriptomics workflow to exploit nasal wash samples from children who tested positive for HRSV. Our analysis revealed a characteristic immune signature as a direct reflection of HRSV pathogenesis and highlighted putative biomarkers of interest such as IP-10, TMEM190, MCEMP1, and TIMM23.